Agilent Cell Analysis and BioTek Laboratory Instrumentation

Integrated solutions for live-cell biology and microplate automation by Agilent Cell Analysis and BioTek: Seahorse metabolic flux analyzers, multimode imaging readers, automated washers, and cytometers.

Agilent Seahorse XF Pro and XFe96 Extracellular Flux Analyzers

Specialized analytical workstations engineered for real-time kinetic measurement of cellular respiration and glycolysis. Systems quantify oxygen consumption rate OCR alongside extracellular acidification rate ECAR across standard 96-well microplates with high reproducibility.

Solid-state sensor cartridges with optical fluorophores detecting oxygen and proton flux
Four automated pneumatic drug injection ports per well for programmable compound delivery
Precision isothermal chamber maintaining uniform thermal equilibrium for cell cultures
Standardized kinetic protocols for mitochondrial stress testing and glycolytic rate profiling

BioTek Cytation 5 and Cytation 7 Cell Imaging Multimode Readers

Automated digital microscopy workstations combining automated imaging optics with multimode microplate detection. Features automated brightfield, phase contrast, and multichannel fluorescence detection paired with variable monochromator optics for comprehensive phenotypic screening.

Motorized objective turret accommodating 1.25x to 60x magnifications with autofocus
Dual imaging engine featuring both inverted and upright microscope optical configurations
Hybrid optical architecture merging Quad monochromators with sensitive bandpass filter cubes
Environmental gas controller regulating carbon dioxide and oxygen for kinetic live-cell assays

BioTek Synergy Neo2 and Synergy H1 Hybrid Multimode Microplate Readers

High-throughput multi-mode microplate readers designed for demanding biochemical screening and cellular assays from 6 to 1536-well density. Incorporates independent optical pathways utilizing variable Quad monochromators alongside high-transmission filter modules for ultra-sensitive readout.

Dedicated photomultiplier tubes delivering high sensitivity for HTRF, TR-FRET, and FP modes
Interchangeable optical filter cubes and high-powered AlphaScreen laser excitation source
Programmable linear and orbital shaking with uniform incubation temperature up to 65 °C
Dual automated reagent syringe injectors facilitating rapid flash luminescence kinetic assays

BioTek LogPhase 600 Microbiology Readers and Epoch 2 Spectrophotometers

Purpose-built absorbance microplate readers engineered for continuous microbial growth curve monitoring at OD600. Instruments deliver simultaneous automated acquisition across four 96-well microplates with robust continuous shaking mechanisms preventing microbial sedimentation.

Parallel automated capacity accommodating up to four standard 96-well plates simultaneously
High-energy orbital shaking mechanism maintaining homogeneous microbial suspension in wells
Integrated Condensation Control thermal gradient preventing droplet formation on plate lids
Monochromator optical selection spanning UV-Vis wavelengths from 200 nm to 999 nm on Epoch 2

BioTek 405 TS and 50 TS Automated Microplate Washers

Industry-benchmark automated washing systems optimized for 96-well and 384-well microplate protocols across ELISA, magnetic bead assays, and adherent cell cultures. Equipped with built-in Ultrasonic Advantage technology preventing manifold needle clogging from crystallized salts.

Integrated ultrasonic water bath automatically clearing manifold dispensing and aspiration pins
Specialized Cell Wash gentle fluidic control minimizing detachment of delicate monolayer cells
Intuitive touchscreen interface programming multi-point bottom aspiration for low residual volumes
Dual-Action wash manifolds supporting independent buffer routing without cross-contamination

BioTek MultiFlo FX Multimode Dispensers and BioSpa 8 Incubators

Versatile automated liquid handling workstations handling dispensing and washing across 6- to 1536-well plate geometries. MultiFlo FX modular architecture accommodates up to four independent dispensing technologies combining peristaltic tubing heads and high-precision syringe drives.

Parallel combination of low dead-volume peristaltic pumps and precision syringe dispensers
Random Access Dispensing RAD technology enabling single-well custom volume liquid addition
Autoclavable dispensing tubing cartridges maintaining sterile conditions for cell growth media
Full hardware synchronization with BioSpa 8 automated incubators and BioStack plate stackers

Agilent xCELLigence Real-Time Cell Analysis (RTCA) Systems

Label-free cell analysis platforms utilizing high-density microelectronic biosensor arrays to monitor cellular phenotypic dynamics. Real-time electrical impedance measurements quantify cell adhesion, proliferation rate, barrier function, and cytotoxicity kinetics without perturbation.

Specialized E-Plate consumables with integrated gold microelectrodes across well bottoms
Label-free non-invasive continuous acquisition preserving authentic cell biology in tests
RTCA DP analyzer architecture featuring three independent 16-well cradles inside incubators
Dual-chamber CIM-Plate consumables quantifying kinetic cell migration and invasion assays

Agilent NovoCyte and NovoCyte Quanteon High-Performance Flow Cytometers

High-performance benchtop flow cytometers engineered for complex multiparameter cellular analysis and immunophenotyping. Equipped with up to four spatially separated solid-state lasers and up to 25 fluorescence detection channels covering a wide dynamic range across diverse cell assays.

Direct volumetric fluidic sampling delivering precise absolute cell counts without beads
Silicon photomultiplier sensors providing high sensitivity with over 7-decade dynamic range
Automated NovoSampler Q accommodating tube racks alongside 96- and 384-well microplate trays
Automated fluidic decontamination and rinsing cycles virtually eliminating sample carryover

Calibration and hydration protocols for Seahorse XF sensor cartridges

Determining cellular oxygen consumption rate OCR and extracellular acidification rate ECAR across Agilent Seahorse XF Pro and XFe96 analyzers requires proper hydration of solid-state sensor cartridges.

Optical fluorophore sensors must be submerged in Seahorse XF Calibrant liquid inside a non-CO2 incubator at thirty-seven degrees for at least eight hours prior to analytical runs.

Loading drug delivery ports with metabolic modulators including Oligomycin, FCCP, and Rotenone demands precision pipetting without touching delicate well walls. Verifying pneumatic manifold seals ensures consistent delivery pressure without fluidic leakage across kinetic runs.

Optical alignment and objective lens upkeep on BioTek Cytation workstations

Automated digital microscopy systems BioTek Cytation 5 and Cytation 7 integrate sensitive optics with high-precision microplate positioning stages.

Preserving imaging resolution across 1.25x to 60x microscope objectives requires routine wiping of front lens elements using spectroscopic-grade alcohol and optical tissue.

Accumulation of culture media vapors on fluorescence filter cubes increases background noise and distorts multi-color image analysis.

Calibrating motorized laser autofocus mechanisms against dedicated validation plates maintains reliable optical focal tracking across long kinetic live-cell experiments.

Optical pathway alignment and monochromator care for Synergy Neo2 readers

Multimode microplate readers BioTek Synergy Neo2 and H1 utilize variable Quad monochromator networks alongside optical bandpass filters for HTRF and TR-FRET assays. Ensuring excitation energy throughput requires periodic inspection of optical mirror alignment and fiber optic light guides.

During high-density screening from 96- to 1536-well microplates, detector compartments remain isolated from chemical vapors. Routine dark current baseline checks against reference verification plates validate photomultiplier linearity across UV and visible wavelengths.

Temperature gradient regulation and condensation control on LogPhase 600

Microbial absorbance readers BioTek LogPhase 600 and Epoch 2 spectrophotometers monitor continuous optical density kinetics of bacterial and fungal suspensions at OD600.

Condensation Control technology maintains an engineered vertical thermal gradient, preventing moisture droplet accumulation on microplate lids during multi-day incubations. Continuous orbital shaking across four standard microplates requires periodic inspection of drive suspension dampers.

Wiping optical lenses free from dust particles prevents false absorbance fluctuations.

Manifold maintenance and ultrasonic decontamination for BioTek 405 TS washers

Operating microplate washers across 405 TS and 50 TS series during ELISA assays involves salt crystallization hazards within fine aspiration and dispensing pins.

Integrated Ultrasonic Advantage cleaning reservoirs require daily deionized water replenishment and regular ultrasonic clearing cycles following operational shifts.

Disassembling Dual-Action manifolds for biochemical residue removal includes thorough inspection of elastomeric O-rings and internal fluid channels. Routine rinsing of vacuum traps and overflow float switches prevents moisture ingress into dry vacuum pump modules.

Peristaltic module calibration and pump upkeep on MultiFlo FX dispensers

BioTek MultiFlo FX multimode dispensers utilize precision peristaltic tubing heads and syringe drives for non-contact micro-dispensing into microplates up to 1536-well density.

To preserve volumetric accuracy, elastomeric pump tubing requires periodic gravimetric volume verification and pump coefficient adjustments. Autoclaving fluidic modules in disassembled configurations protects tubing elasticity against thermal micro-cracking.

Wiping RAD dispensing pins removes protein deposits and avoids droplet deflection during low-volume liquid transfers into individual wells.

Microelectrode biosensor verification on xCELLigence RTCA workstations

Label-free impedance analysis across Agilent xCELLigence RTCA DP and RTCA eSight systems depends on the electrical integrity of gold biosensors in E-Plate consumables.

Prior to experimental setup, recording baseline electrical impedance across buffer-filled wells verifies spring-loaded gold contact pins on incubation station cradles.

Ribbon interconnect cables routing signals between the incubator pod and the external analyzer controller must remain free from sharp bends or thermal stress. Wiping station contact pins with isopropyl alcohol prevents high-resistance contact artifacts.

Fluidic system decontamination and laser alignment on NovoCyte flow cytometers

Detection sensitivity and volumetric precision across Agilent NovoCyte and NovoCyte Quanteon flow cytometers require laminar hydrodynamic flow within the quartz flow cell.

Routine fluidic decontamination using NovoClean reagent followed by deionized NovoRinse flushes prevents fluorochrome dye carryover and cell debris build-up in sample intake needles. Monitoring sheath fluid reservoir pressure ensures uninterrupted hydrodynamic focusing during acquisition batches.

Periodic calibration with fluorescent alignment beads confirms stable detector amplification factors and spectral separation.

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