Chromatography Resins, Sorbents and Columns Suzhou NanoMicro Technology / NanoMicro

Engineering selection and supply of Suzhou NanoMicro Technology / NanoMicro chromatography media: UniMab affinity resins, NanoGel polymeric ion exchangers, UniSil silica sorbents, and prepacked columns.

NanoMicro UniMab and NMab Protein A Affinity Chromatography Resins

UniMab and NMab polymer affinity resins feature alkali-tolerant recombinant Protein A for monoclonal antibody capture. The monodisperse rigid matrix tolerates repeated CIP sanitization with 0.1–0.5 M NaOH. Resins deliver high dynamic binding capacity and sharp elution profiles.

Affinity resins UniMab, UniMab Pro, and NMab with Protein A ligand
Primary capture of monoclonal antibodies IgG, Fc-fusions, and fragments
Dynamic binding capacity up to 60 mg/mL at 4-6 minutes residence time
Alkaline stability across over 200 CIP cleaning cycles with NaOH

NanoMicro NanoGel Polymeric Ion Exchange Chromatography Resins

NanoGel ion exchange resins with SP, CM, Q, and DEAE functional chemistries are built on hydrophilic monodisperse polymers. Media enable intermediate purification and polishing of therapeutic proteins, plasmid DNA, and viral antigens at high preparative linear flow velocities.

Strong and weak ion exchangers NanoGel-SP, NanoGel-CM, Q, and DEAE
Intermediate purification of recombinant proteins and viral vectors
Monodisperse beads in 30 and 50 um particle sizes with pore control
Chemical stability from pH 2 to 14 with high mechanical resistance

NanoMicro UniSil Monodisperse Spherical Silica Chromatography Media

UniSil spherical silica media feature uniform particle sizes of 10, 15, 30, and 50 um for reversed-phase and normal-phase separations. UniSil C18 and bare silica provide rigid pore networks, delivering high loading capacity for peptides, antibiotics, and active natural products.

Silica bulk media UniSil C18, C8, C4, HILIC, and bare silica
Preparative fractionation of synthetic peptides and natural products
Controlled pore sizes 100, 120, 200, and 300 A with narrow deviation
Rigid bead architecture engineered for DAC column bed stability

NanoMicro UniHIC Hydrophobic Interaction and Multimodal Resins

UniHIC hydrophobic interaction resins (Butyl, Phenyl, Ether) and UniMMS multimodal media separate proteins by combined charge and hydrophobic affinity. Media are tailored for antibody-drug conjugates (ADCs), recombinant vaccines, and protein charge variants without conformational loss.

Hydrophobic resins UniHIC-Butyl, Phenyl, Ether and multimodal UniMMS
Separation of antibody-drug conjugates (ADCs) and complex vaccines
Mild salt elution preserving native target protein conformations
Hydrophilic crosslinked polymer matrix stable between pH 2 and 12

NanoMicro Prepacked Chromatography Columns and Screening Formats

Prepacked Columns are packed with proprietary UniSil, UniGel, or UniMab media under strict factory conditions. Hardware delivers reproducible retention times, high peak symmetry, and lot-to-lot reliability when screening parameters and scaling up biopharmaceutical purification methods.

Prepacked formats NMTrap, NMScreen, NMValid, and preparative scale columns
Chromatographic condition screening, scale-down modeling, and validation
Hardware volumes from 1 to 50 mL and bed lengths from 50 to 250 mm
Individual certificate of analysis (CoA) and efficiency test report

NanoMicro Chromatography Column Hardwares and Distribution Spares

Column Hardwares and sanitary distribution spares are engineered from 316L stainless steel, titanium, and PEEK polymers. Assemblies feature adjustable pistons, uniform distributor frits, and sanitary fittings to maintain dense packed bed geometry at pressures up to 10–30 MPa.

High and medium pressure column hardwares NMXK and preparative tubes
Stable bed confinement for silica and polymeric media in preparative LC
Wetted contact surfaces built in 316L, titanium, and medical PEEK
Porous titanium distribution frits, PTFE seals, and sanitary adapters

Column Packing and Bed Stabilization Protocol for UniSil Monodisperse Silica Media

When preparing preparative chromatography columns with UniSil monodisperse silica media, achieving uniform packed bed density is critical for separation efficiency. Dry media should be slurried in a mixture of isopropanol or methanol with deionized water in a one to two volumetric ratio.

Slurry concentration is maintained around forty percent by volume, avoiding excessive high-shear mechanical agitation that could fracture particles.

Packing is conducted using dynamic axial compression (DAC) or a high-pressure slurry packing pump at twenty percent above maximum anticipated operating pressure. The uniform hydrodynamic settling velocity of monodisperse microspheres eliminates particle size segregation and channeling.

Following bed consolidation, the column is conditioned with five column volumes of mobile phase to verify theoretical plate counts and peak asymmetry.

Clean-in-Place (CIP) Sanitization and Alkaline Regeneration of UniMab Affinity Resins

To maintain microbiological control and restore binding capacity of UniMab and NMab affinity resins, periodic clean-in-place (CIP) protocols are implemented.

The crosslinked monodisperse polymer backbone coupled with engineered recombinant Protein A withstands alkaline sodium hydroxide solutions between 0.1 and 0.5 M. Prior to alkaline exposure, weakly bound impurities are stripped using high ionic strength wash buffers.

Sodium hydroxide solution is pumped at reduced linear flow velocity, maintaining a minimum residence contact time of fifteen minutes. Following CIP exposure, the bed is re-equilibrated with neutral phosphate buffer until effluent pH stabilizes at seven.

This regimen removes non-specifically bound host cell proteins (HCP) and nucleic acid fragments while conserving specific ligand binding affinity.

Ion Exchange Chromatography Optimization and Step Elution on NanoGel Polymeric Resins

Preparative biomolecule separation on NanoGel hydrophilic polymer ion exchangers (SP, CM, Q, DEAE) depends on optimized ionic strength gradients and buffer capacity.

During sample loading, feed conductivity is lowered through diafiltration or dilution to promote complete electrostatic binding of target fractions to charged functional groups on the matrix.

Elution is executed via step or linear sodium chloride gradients spanning from zero to one mole per liter. The rigid macroporous architecture of NanoGel monodisperse polymer beads prevents bed shrinkage or swelling during steep ionic strength shifts.

This bed stability prevents excessive hydraulic resistance and produces sharp chromatographic elution peaks at high mass loading.

Engineering Selection of NanoMicro Chromatography Media via Technical Data Sheets (TDS)

Selecting optimal NanoMicro chromatography media requires evaluating molecular weight, hydrophobicity, and isoelectric point of target compounds.

For recombinant monoclonal antibodies, UniMab Protein A affinity resins serve as the primary capture phase, delivering purity above ninety-five percent in a single downstream step. For synthetic peptide purification, UniSil C18 with 100 or 120 angstrom pores is preferred.

Engineers review manufacturer technical data sheets (TDS), chemical compatibility profiles, and safety data sheets (SDS).

Based on customer purification requirements, required bed volumes, slurry volume allowances, and batch supply delivery schedules are calculated with full technical documentation support.

Suzhou NanoMicro Technology / NanoMicro and model names are trademarks of their respective owners and are used only to identify the equipment for which we source parts. SpareX is not an official dealer, distributor or representative of Suzhou NanoMicro Technology / NanoMicro and is not affiliated with the trademark owner. Origin, documents and warranty are stated for each item in the quotation.